WRKY22 and WRKY25 transcription factors are positive regulators of defense responses in Nicotiana benthamiana.
Identifieur interne : 000007 ( Main/Exploration ); précédent : 000006; suivant : 000008WRKY22 and WRKY25 transcription factors are positive regulators of defense responses in Nicotiana benthamiana.
Auteurs : Romina N. Ramos [Argentine] ; Gregory B. Martin [États-Unis] ; Marina A. Pombo [Argentine] ; Hernan G. Rosli [Argentine]Source :
- Plant molecular biology [ 1573-5028 ] ; 2020.
Abstract
KEY MESSAGE
NbWRKY22 and NbWRKY25 are required for full activation of bacteria-associated pattern- and effector-triggered immunity as well as for the response to other non-bacterial defense elicitors. Plants defend themselves against pathogens using a two-layered immune system. Pattern-triggered immunity (PTI) can be activated upon recognition of epitopes from flagellin including flg22. Pseudomonas syringae pv. tomato (Pst) delivers effector proteins into the plant cell to promote host susceptibility. However, some plants express resistance (R) proteins that recognize specific effectors leading to the activation of effector-triggered immunity (ETI). Resistant tomato lines such as Rio Grande-PtoR (RG-PtoR) recognize two Pst effectors, AvrPto and AvrPtoB, and activate ETI through the Pto/Prf protein complex. Using RNA-seq, we identified two tomato WRKY transcription factor genes, SlWRKY22 and SlWRKY25, whose expression is increased during Pst-induced ETI. Silencing of the WRKY25/22 orthologous genes in Nicotiana benthamiana led to a delay in programmed cell death normally associated with AvrPto recognition or several non-bacterial effector/R protein pairs. An increase in disease symptoms was observed in silenced plants infiltrated with Pseudomonas syringae pv. tabaci expressing AvrPto or HopQ1-1. Expression of both tomato WRKY genes is also induced upon treatment with flg22 and callose deposition and cell death suppression assays in WRKY25/22-silenced N. benthamiana plants supported their involvement in PTI. Our results reveal an important role for two WRKYs as positive regulators of plant immunity against bacterial and potentially non-bacterial pathogens.
DOI: 10.1007/s11103-020-01069-w
PubMed: 32909182
Affiliations:
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Pombo</name><affiliation wicri:level="1"><nlm:affiliation>INFIVE, Instituto de Fisiología Vegetal, Universidad Nacional de La Plata, CONICET, La Plata, Buenos Aires, Argentina. mpombo@agro.unlp.edu.ar.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>INFIVE, Instituto de Fisiología Vegetal, Universidad Nacional de La Plata, CONICET, La Plata, Buenos Aires</wicri:regionArea><wicri:noRegion>Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Rosli, Hernan G" sort="Rosli, Hernan G" uniqKey="Rosli H" first="Hernan G" last="Rosli">Hernan G. Rosli</name><affiliation wicri:level="1"><nlm:affiliation>INFIVE, Instituto de Fisiología Vegetal, Universidad Nacional de La Plata, CONICET, La Plata, Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>INFIVE, Instituto de Fisiología Vegetal, Universidad Nacional de La Plata, CONICET, La Plata, Buenos Aires</wicri:regionArea><wicri:noRegion>Buenos Aires</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant molecular biology</title><idno type="eISSN">1573-5028</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>KEY MESSAGE</b></p><p>NbWRKY22 and NbWRKY25 are required for full activation of bacteria-associated pattern- and effector-triggered immunity as well as for the response to other non-bacterial defense elicitors. Plants defend themselves against pathogens using a two-layered immune system. Pattern-triggered immunity (PTI) can be activated upon recognition of epitopes from flagellin including flg22. Pseudomonas syringae pv. tomato (Pst) delivers effector proteins into the plant cell to promote host susceptibility. However, some plants express resistance (R) proteins that recognize specific effectors leading to the activation of effector-triggered immunity (ETI). Resistant tomato lines such as Rio Grande-PtoR (RG-PtoR) recognize two Pst effectors, AvrPto and AvrPtoB, and activate ETI through the Pto/Prf protein complex. Using RNA-seq, we identified two tomato WRKY transcription factor genes, SlWRKY22 and SlWRKY25, whose expression is increased during Pst-induced ETI. Silencing of the WRKY25/22 orthologous genes in Nicotiana benthamiana led to a delay in programmed cell death normally associated with AvrPto recognition or several non-bacterial effector/R protein pairs. An increase in disease symptoms was observed in silenced plants infiltrated with Pseudomonas syringae pv. tabaci expressing AvrPto or HopQ1-1. Expression of both tomato WRKY genes is also induced upon treatment with flg22 and callose deposition and cell death suppression assays in WRKY25/22-silenced N. benthamiana plants supported their involvement in PTI. Our results reveal an important role for two WRKYs as positive regulators of plant immunity against bacterial and potentially non-bacterial pathogens.</p></div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32909182</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-5028</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Sep</Month><Day>09</Day></PubDate></JournalIssue><Title>Plant molecular biology</Title><ISOAbbreviation>Plant Mol Biol</ISOAbbreviation></Journal><ArticleTitle>WRKY22 and WRKY25 transcription factors are positive regulators of defense responses in Nicotiana benthamiana.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11103-020-01069-w</ELocationID><Abstract><AbstractText Label="KEY MESSAGE" NlmCategory="UNASSIGNED">NbWRKY22 and NbWRKY25 are required for full activation of bacteria-associated pattern- and effector-triggered immunity as well as for the response to other non-bacterial defense elicitors. Plants defend themselves against pathogens using a two-layered immune system. Pattern-triggered immunity (PTI) can be activated upon recognition of epitopes from flagellin including flg22. Pseudomonas syringae pv. tomato (Pst) delivers effector proteins into the plant cell to promote host susceptibility. However, some plants express resistance (R) proteins that recognize specific effectors leading to the activation of effector-triggered immunity (ETI). Resistant tomato lines such as Rio Grande-PtoR (RG-PtoR) recognize two Pst effectors, AvrPto and AvrPtoB, and activate ETI through the Pto/Prf protein complex. Using RNA-seq, we identified two tomato WRKY transcription factor genes, SlWRKY22 and SlWRKY25, whose expression is increased during Pst-induced ETI. Silencing of the WRKY25/22 orthologous genes in Nicotiana benthamiana led to a delay in programmed cell death normally associated with AvrPto recognition or several non-bacterial effector/R protein pairs. An increase in disease symptoms was observed in silenced plants infiltrated with Pseudomonas syringae pv. tabaci expressing AvrPto or HopQ1-1. Expression of both tomato WRKY genes is also induced upon treatment with flg22 and callose deposition and cell death suppression assays in WRKY25/22-silenced N. benthamiana plants supported their involvement in PTI. Our results reveal an important role for two WRKYs as positive regulators of plant immunity against bacterial and potentially non-bacterial pathogens.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ramos</LastName><ForeName>Romina N</ForeName><Initials>RN</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-2991-2153</Identifier><AffiliationInfo><Affiliation>INFIVE, Instituto de Fisiología Vegetal, Universidad Nacional de La Plata, CONICET, La Plata, Buenos Aires, Argentina.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martin</LastName><ForeName>Gregory B</ForeName><Initials>GB</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-0044-6830</Identifier><AffiliationInfo><Affiliation>Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, NY, 14853, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pombo</LastName><ForeName>Marina A</ForeName><Initials>MA</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-4057-2700</Identifier><AffiliationInfo><Affiliation>INFIVE, Instituto de Fisiología Vegetal, Universidad Nacional de La Plata, CONICET, La Plata, Buenos Aires, Argentina. mpombo@agro.unlp.edu.ar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rosli</LastName><ForeName>Hernan G</ForeName><Initials>HG</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-3929-5630</Identifier><AffiliationInfo><Affiliation>INFIVE, Instituto de Fisiología Vegetal, Universidad Nacional de La Plata, CONICET, La Plata, Buenos Aires, Argentina.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>PICT2014-1589</GrantID><Agency>Agencia Nacional de Promoción Científica y Tecnológica</Agency><Country></Country></Grant><Grant><GrantID>PICT2017-0916</GrantID><Agency>Agencia Nacional de Promoción Científica y Tecnológica</Agency><Country></Country></Grant><Grant><GrantID>PIP2014-0314</GrantID><Agency>Consejo Nacional de Investigaciones Científicas y Técnicas (AR)</Agency><Country></Country></Grant><Grant><GrantID>PUE INFIVE 2016-0110</GrantID><Agency>Consejo Nacional de Investigaciones Científicas y Técnicas (AR)</Agency><Country></Country></Grant><Grant><GrantID>IOS-1546625</GrantID><Agency>National Science Foundation (USA)</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Plant Mol Biol</MedlineTA><NlmUniqueID>9106343</NlmUniqueID><ISSNLinking>0167-4412</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bacterial speck disease</Keyword><Keyword MajorTopicYN="N">Plant immunity</Keyword><Keyword MajorTopicYN="N">Pseudomonas syringae pv. tomato</Keyword><Keyword MajorTopicYN="N">WRKY transcription factors</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>10</Day><Hour>5</Hour><Minute>38</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32909182</ArticleId><ArticleId IdType="doi">10.1007/s11103-020-01069-w</ArticleId><ArticleId IdType="pii">10.1007/s11103-020-01069-w</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Argentine</li><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><country name="Argentine"><noRegion><name sortKey="Ramos, Romina N" sort="Ramos, Romina N" uniqKey="Ramos R" first="Romina N" last="Ramos">Romina N. Ramos</name></noRegion><name sortKey="Pombo, Marina A" sort="Pombo, Marina A" uniqKey="Pombo M" first="Marina A" last="Pombo">Marina A. Pombo</name><name sortKey="Ramos, Romina N" sort="Ramos, Romina N" uniqKey="Ramos R" first="Romina N" last="Ramos">Romina N. Ramos</name><name sortKey="Rosli, Hernan G" sort="Rosli, Hernan G" uniqKey="Rosli H" first="Hernan G" last="Rosli">Hernan G. Rosli</name></country><country name="États-Unis"><noRegion><name sortKey="Martin, Gregory B" sort="Martin, Gregory B" uniqKey="Martin G" first="Gregory B" last="Martin">Gregory B. Martin</name></noRegion><name sortKey="Martin, Gregory B" sort="Martin, Gregory B" uniqKey="Martin G" first="Gregory B" last="Martin">Gregory B. Martin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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